eea1 antibody (Cell Signaling Technology Inc)
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Eea1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eea1+antibody/EEA1+Antibody/pmc13018965-41-15-17
Average 96 stars, based on 771 article reviews
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Immunoprecipitation:Article Title: P-Rex1 limits glucose clearance and suppresses hepatocyte glucose uptake and mitochondrial metabolism independently of its Rac-GEF activity. Article Snippet: .. Whole endosomes were immunoprecipitated from the rest of the 10k sup as described, 38,39 by incubation with Article Title: Prex1 controls glucose homeostasis by limiting glucose uptake and mitochondrial metabolism in liver through GEF-independent regulation of Gpr21 Article Snippet: .. 950 μl of the supernatant were transferred into a fresh, precooled tube, and whole endosomes were immunoprecipitated as described , by incubation with Incubation:Article Title: P-Rex1 limits glucose clearance and suppresses hepatocyte glucose uptake and mitochondrial metabolism independently of its Rac-GEF activity. Article Snippet: .. Whole endosomes were immunoprecipitated from the rest of the 10k sup as described, 38,39 by incubation with Article Title: Prex1 controls glucose homeostasis by limiting glucose uptake and mitochondrial metabolism in liver through GEF-independent regulation of Gpr21 Article Snippet: .. 950 μl of the supernatant were transferred into a fresh, precooled tube, and whole endosomes were immunoprecipitated as described , by incubation with Article Title: Disrupting Notch signalling by a small molecule inhibiting dihydroorotate dehydrogenase activity Article Snippet: .. Cells were incubated with primary Blocking Assay:Article Title: Protocol for monitoring the endosomal trafficking of membrane proteins in mammalian cells Article Snippet: .. Fix the cells with 500 μL of 4% PFA at room temperature for 15 min. c. Wash off the excess PFA with PBS. d. Permeabilize the cells using 500 μL of 0.1% NP-40 in PBS for 10 min at room temperature. e. After permeabilization, wash the cells with PBS three times to remove excess permeabilization buffer. f. Block the cells with 500 μL of 3% BSA in PBS for 1 h at room temperature. g. Dilute the Article Title: Protocol for monitoring the endosomal trafficking of membrane proteins in mammalian cells. Article Snippet: .. Fix the cells with 500 mL of 4% PFA at room temperature for 15 min. c. Wash off the excess PFA with PBS. d. Permeabilize the cells using 500 mL of 0.1% NP-40 in PBS for 10 min at room temperature. e. After permeabilization, wash the cells with PBS three times to remove excess permeabiliza- tion buffer. f. Block the cells with 500 mL of 3% BSA in PBS for 1 h at room temperature. g. Dilute the Staining:Article Title: Bioengineered protein nanocarrier facilitating siRNA escape from lysosomes for targeted RNAi therapy in glioblastoma Article Snippet: .. To study the colocalization of these formulations with endosomes, other:Article Title: A rapid and reliable strategy for identification of LNP and protein corona in vitro and in vivo Article Snippet: Water, methanol, acetonitrile, isopropanol (Fisher Scientific, New Hampshire, USA); BeyoBlueTM Plus (Beyotime Biotechnology, Shanghai, China); |


